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binding buffer cell apoptosis kit  (MBL Life science)

 
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    MBL Life science binding buffer cell apoptosis kit
    Binding Buffer Cell Apoptosis Kit, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+buffer+cell+apoptosis+kit/binding+buffer+cell+apoptosis+kit/pmc07934477-293-18-21
    Average 90 stars, based on 1 article reviews
    binding buffer cell apoptosis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Pluripotent stem cell derived models of neurological diseases reveal early transcriptional heterogeneity
    Article Snippet: .. Cells were pelleted at 1200 RPM (300 g) for 10 min, and re-suspended in Binding buffer from the cell apoptosis kit (MBL, code: 4700). .. AnnexinV-FITC, PI (MBL, code: 4700) and ANTI-PSA-NCAM conjugated-Ab (Miltenyi Biotec, cat. 130-093-273) were added according to the manufacturer’s instructions.

    Article Title: Pluripotent stem cell-derived models of neurological diseases reveal early transcriptional heterogeneity
    Article Snippet: .. Cells were pelleted at 1200 RPM (300 g) for 10 min, and re-suspended in Binding buffer from the cell apoptosis kit (MBL, code: 4700). .. AnnexinV-FITC, PI (MBL, code: 4700), and ANTI-PSA-NCAM conjugated-Ab (Miltenyi Biotec, cat. 130-093-273) were added according to the manufacturer’s instructions.



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    Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the apoptosis of human non-small cell lung cancer A549 cell line. *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Control

    Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Hoechst 33342 staining for the effect of taurine on the apoptosis of cell line A549 (magnification, ×200). *P<0.05, **P<0.01 vs. control group. Tau, taurine; DDP, cisplatin.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Staining, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 proteins in human lung cancer A549 cell line. Values are expressed as the mean ± standard deviation; n=3. *P<0.05 vs. control group. Tau, taurine; DDP, cisplatin; Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis; IOD, integrated option density.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Standard Deviation, Control

    Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Suppressive effect of taurine and PUMA expression on the growth of lung cancer A549 cell-derived xenograft tumors in nude mice. (A) The growth curve of tumor in nude mice bearing human non-small cell lung cancer A549 cell xenografts. (B) Tumors in nude mice bearing human non-small cell lung cancer A549 cells xenografts. (C) The tumor inhibition rate following 27 days of treatment. Values are expressed as the mean ± SD; n=5. **P<0.01 vs. negative or control; # P<0.05 vs. the PUMA or taurine group. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Inhibition, Control

    Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters expression of PUMA in lung cancer A549 cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Taurine alters the expression of Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice (magnification, ×400). (A) Control; (B) negative; (C) exogenous PUMA; (D) taurine; (E) taurine and exogenous PUMA. Tau, taurine; PUMA, p53 upregulated modulator of apoptosis; Bcl-2, BCL2, apoptosis regulator.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control

    Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Journal: Oncology Letters

    Article Title: Effect of taurine on cell proliferation and apoptosis human lung cancer A549 cells

    doi: 10.3892/ol.2018.8036

    Figure Lengend Snippet: Effect of taurine on the expression of PUMA, Bax and Bcl-2 in A549 lung cancer cell-derived xenograft tumors in nude mice. **P<0.01 vs. control or negative; # P<0.01 vs. PUMA or taurine (mean ± standard deviation; n=5). Bcl-2, BCL2 apoptosis regulator; Bax, BCL2 associated X, apoptosis regulator; PUMA, p53 upregulated modulator of apoptosis.

    Article Snippet: Following a 48 h treatment, as aforementioned, 1–5×10 5 cells were collected and resuspended in 500 μl Binding Buffer (Annexin V-FITC/PI double staining cell apoptosis detection kit; Jiangsu Keygen Biotechnology Co., Ltd., Nanjing, China).

    Techniques: Expressing, Derivative Assay, Control, Standard Deviation